Introduction to M3Drop: Michaelis-Menten modelling of dropouts in scRNASeq
نویسنده
چکیده
Single-cell RNA sequencing is able to quantify the whole transcriptome from the small amount of RNA present in individual cells. However, a consequence of reverse-transcribing and amplifying small quantities of RNA is a large number of dropouts, genes with zero expression in particular cells. The frequency of dropout events is strongly non-linearly related to the measured expression levels of the respective genes. M3Drop posits that these dropouts are due to failures of reverse transcription, a simple enzyme reaction, thus should be modelled using the Michaelis-Menten equation as follows:
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